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NPN Protocol Details Image

Hackelia (venusta)

Anthony S. Davis
Director and Assistant Professor
University of Idaho - Center for Forest Nursery and Seedling Research
University of Idaho, PO Box 441137
Moscow, Idaho 83844-1137
208.885.3888
208.885.6226 (fax)
asdavis@uidaho.edu
http://seedlings.uidaho.com/

Family Scientific Name: Boraginaceae
Family Common Name: Borage family
Scientific Name: Hackelia venusta (Piper) St. John
Common Name: Showy stickseed
Species Code: HAVE
Ecotype: Dry, eastern slopes of Cascade Mtn range with ponderosa pine.
General Distribution: This species of Hackelia is only found in Chelan County in the Washington Cascade Mountain Range.
Propagation Goal: plants
Propagation Method: vegetative
ProductType: Container (plug)
Stock Type: 165 ml capacity Hillson Rootrainers
Time To Grow: 16 Weeks
Target Specifications: 15-cm-long, firm root plug
Propagule Collection: Propagules were rooted microshoots.
Pre-Planting Treatments: Rooted propagules were removed from sterile, glass tubes. The roots were gently washed under running water to remove any remaining agar.
Establishment Phase: Rooted propagules were planted in rootrainers containing a 1:1 volume of Sunshine peat and perlite. Relative humidity was 90% and progressively decreased to ambient over the course of 4 weeks. Plantlets were kept in 70% shade and slowly acclimated to normal greenhouse sunlight over 4 weeks.
Length of Establishment Phase: 1 month
Active Growth Phase: Acclimated plantlets were then fertilized with 20:20:20 (N:P2O5:K2O) at 200 ppm N twice a week for 8 weeks.
Length of Active Growth Phase: 2 months
References: Edson, JL., Leege-Brusven, A., Everett, RL., and Wenny, DL. 1996. Minimizing growth regulators in shoot culture of an endangered plant, Hackelia venusta (Boraginaceae). In Vitro Cell. Dev. Biol. - Plants 32:267-271.

Citation:

Brusven, Annette D.L.; Edson, John L.; Everett, Richard L.; Wenny, David L. 2001. Propagation protocol for production of Container (plug) Hackelia venusta (Piper) St. John plants 165 ml capacity Hillson Rootrainers; University of Idaho - Center for Forest Nursery and Seedling Research Moscow, Idaho. In: Native Plant Network. URL: https://NativePlantNetwork.org (accessed 2024/07/23). US Department of Agriculture, Forest Service, National Center for Reforestation, Nurseries, and Genetic Resources.