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U.S. Department of Agriculture USDA Forest Service Southern Regional Extension Forestry Southern Regional Extension Forestry

A Genotype-Independent Method for Transformation of Pines Using Agrobacterium

Forest products constitute the third most important cash crop in Texas after cotton and grain sorghum. Of the forest species, loblolly pine is the most important. The single most important factor blocking improvement of loblolly and other commercial pine genotypes through genetic engineering has been an inability to regenerate plants following transformation. To overcome this block in plant regeneration, we modified a direct shoot apex inoculation procedure using Agrobacterium, developed earlier for cotton and maize (Gould et al., 1991).


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Author(s): J. Gould, Y. Zhou, M. E. Magallanes-Cedeno, Y. Shen, V. Padmanabhan, Ronald J. Newton

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 1997

Section: Contributed Presentations: Extended Abstracts