Factors regulating asexual embryogensis in vitro in longleaf pine
Embryogenic cultures of longleaf pine (Pinus palustris Mill.) were established from female gametophytes with intact zygotic embryos (at developmental stages 1 and 2) cultured on MSG and DCR basal media in darkness. Induction of embryogenic tissue was observed from explants cultured on both basal media containing glucose, or maltose or sucrose as carbon source. All four levels of each carbon source (15-90 g/L) used supported production of embryogenic tissue.
Download this file:
Download this file — PDF document, 261KbDetails
Author(s): R. Nagmani, Alex M. Diner, G. C. Sharma
Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 1993
Section: Concurrent Session 3: Softwood Propagation