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Home → Publications → Tree Improvement and Genetics → Southern Forest Tree Improvement Conference → 20th Southern Forest Tree Improvement Conference (1989) → Development of In Vitro and In Vivo Screening Techniques to Detect Littleleaf Disease Resistance

Development of In Vitro and In Vivo Screening Techniques to Detect Littleleaf Disease Resistance

Two-month-old sand cultured pine seedlings were inoculated with zoospores and chlamydospores of Phytophthora cinnamomi Rands to determine in vivo susceptibility. Seedlings from sand culture were harvested and gently washed with tap water to remove sand particles. Root tips of the intact seedlings were then inoculated with differing dosages of zoospores or chlamydospores of P. cinnamomi in a moist chamber by using a micropipette inoculation technique. Two, five, and ten spores per microliter were used as inoculum dosage treatments.


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Author(s): J. C. Jang, F. H. Tainter

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 1989

Section: Poster Abstracts