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Home → Publications → Tree Improvement and Genetics → Southern Forest Tree Improvement Conference → 19th Southern Forest Tree Improvement Conference (1987) → Induction of New Host Coded Proteins in Pinus elliottii Seedlings in Response to Pathogen and Water Stress

Induction of New Host Coded Proteins in Pinus elliottii Seedlings in Response to Pathogen and Water Stress

A 55 kD stress protein was induced in tissue culture grown slash pine seedlings infected with conidial suspensions of pitch canker fungus. Induction was evident in both hypocotyl and root sections of the seedlings after a lag phase of 16 hours. The stress protein is best extracted from stressed tissues with slightly alkaline Tris-HCI (pH 8.65) buffer. We have also investigated protein synthesis in water-stressed slash pipe seedlings. Mannitol-induced water stress inhibited overall protein synthesis, with induction of unique stress proteins in the hypocotyl tissue. At -1.8 MPa a 46 kD stress protein was induced. At -2.5 MPa at least five stress proteins 82 kD, 71 kD, 70 kD, 64 kD and 58 kD were detected by SDS-PAGE and Fluorography.


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Author(s): J. V. Valluri, E. J. Soltes, Ronald J. Newton, B. G. Cobb

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 1987