In Vitro Propagation of Prunus Serotina

Scions from mature Prunus serotina trees were grafted onto seedling root stocks and grown in a greenhouse for approximately five years. Lateral buds were excised just as the buds were beginning to swell. Bud elongation was initiated on Murashige and Skoog inorganic salts, modified with 100 mg/liter i-Inositol, 0.4 mg/liter thiamine-HCL, LO mg/liter N-6-benzylaminopurine (BA) , 0.1 mg/liter gibberillic acid (GA 3 ), 0.1 mg/liter indole-3- butyric acid (IBA), 2% sucrose, and 7.0 gms/liter phytagar. Buds elongated in 2 to 4 weeks. Adventicious shoot multiplication was induced on the same basal medium as above, modified with 0.75 mg/liter BA, 0.2 mg/-liter GA3, and 0.01 mg/liter IBA. The sucrose concentration was increased to 3%. An average shoot multiplication rate of 5 to 1 was achieved every 3 to 4 weeks. Root formation was induced in vitro on half strength Murashige and Skoog inorganic salts, 100 mg/liter i-Inositol, 0.4 mg/-liter liter thiamine-HCL, 1.0 mg/liter IBA, 2% sucrose, and 7.0 gms/liter phytagar. Rooting was significantly enhanced by incubating the cultures in continuous darkness. Roots develop in 7 to 10 days.

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Author(s): D. M. Tricoli

Publication: Tree Improvement and Genetics - Northeastern Forest Tree Improvement Conference - 1982

Section: Session 4