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Home → Publications → Tree Planters' Notes → Tree Planters' Notes Volume 44, Number 4 (1993) → Genotyping of Longleaf Pine Ramets After Hurricane Hugo by Using DNA and Isozyme Markers

Genotyping of Longleaf Pine Ramets After Hurricane Hugo by Using DNA and Isozyme Markers

Isozyme and restriction fragment length polymorphism (RFLP) markers were used to determine the genetic identities of 12 longleaf pine (Pinus palustris Mill.) ramets whose identities came into question after Hurricane Hugo. Isozyme assays were performed for 12 enzyme systems representing 15 loci. Variation at 6 loci revealed unique identities for 6 ramets. Four RFLP probes showed that 9 of the 12 ramets were genetically unique. This study confirms the power of isozyme analysis for determining genetic identity of seed orchard clones and, in addition, demonstrates the increased power of genetic discrimination offered by RFLP analysis.


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Author(s): David B. Neale, E. R. Carroll, Kathleen D. Jermstad, P. A. Guge, S. T. Friedman

Publication: Tree Planters' Notes - Volume 44, Number 4 (1993)

Volume: 44

Number: 4