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Home → Publications → Tree Improvement and Genetics → Southern Forest Tree Improvement Conference → 31st Southern Forest Tree Improvement Conference (2011) → Genetic variation of Japanese elm (Ulmus davidiana var. japonica) employing ISSR markers

Genetic variation of Japanese elm (Ulmus davidiana var. japonica) employing ISSR markers

We studied genetic variation of Japanese elm calling medicinal tree, Ulmus davidiana var. japonica (Rehder) Nakai, to establish the strategy for the conservation of its genetic resources. A total of 171 individual samples were collected from seven populations and their DNAs were used for inter simple sequence repeat-PCR amplification with 47 ISSR primers. Eight out of the 47 primers were selected and yielded 55 clear fragments to be scored. The percentage of polymorphic loci (P) ranged from 87.27% to 98.18% with a mean of 93.25%. The gene diversity (h) averaged over all was 0.33. The Shannon’s information index (S.I.) ranged between 0.455 and 0.515 with an average of 0.494. The AMOVA showed that most of the genetic variation (96.1%) was allocated among individuals within populations. The dendrogram showed no clear association between the clustering of population and their geographical origin.


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Author(s): Joon-Hyeok Kim, Byeong-Hoon Yang, Jin-Taek Kang, Yong-Pyo Hong