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Home → Publications → Tree Improvement and Genetics → Southern Forest Tree Improvement Conference → 30th Southern Forest Tree Improvement Conference (2009) → Chromosomal Distribution of Repetitive DNA Sequences in Pine using Fluorescent In Situ Hybridization (FISH)

Chromosomal Distribution of Repetitive DNA Sequences in Pine using Fluorescent In Situ Hybridization (FISH)

The genus Pinus (pines, 2n = 2x = 24) includes many economically and ecologically important species worldwide. Pine genomes are exceptionally large with 1C (i.e., haploid DNA) contents ranging from 20 to 30 billion base pairs (Wakamiya et al. 1993; Bogunic et al. 2003). Much of this DNA content consists of high numbers of copia- and gypsy-like long terminal repeat (LTR) retrotransposons. A few of these retrotransposons have been formally described in conifers (Kamm et al. 1996; Kossack and Kinlaw 1999; Friesen et al. 2001; Rocheta et al. 2007; Morse et al. 2009), although only one (Gymny, Morse et al. 2009) has been characterized using fluorescent in situ hybridization (FISH). Here we present initial FISH-based characterization of 32 newly isolated repetitive pine DNA sequences.


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Author(s): C. Dana Nelson, Daniel G. Peterson, M. Abdul Majid, Nurul Islam-Faridi, Z. V. Magbanua

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 2009