Isolation And Characterization Of Water Deficit Stress Inducible Cdnas And Their Genomic Counterparts From Pinus Taeda (loblollypine)

Water deficit stress (WDS) is one of the most important factors affecting trees in forest stands. To survive the stress, plants activate certain genes of which the translated products are assumed to play a major role in tolerance. Previously we have cloned and characterized 3 genes. Here we describe another WDS induced gene. The predicted translation product of 1p5 is rich in glycine (40%) and serine (20%), and appears to be a cell wall targeted protein with a possible function in cell wall reinforcement. In order to understand the function in vivo, 1p5 gene has been cloned behind a CaMV 35S promoter and used in transformation studies. Also the genomic clones of 1p3 and 1p5 genes were isolated from a lambda library and sequence analysis was done. A 1.1 kb fragment of 1p3 and 2.3 kb piece of lp5 upstream of the transcription start site have been cloned in front of gus genes and these constructs have been used in the transformation of tobacco. Preliminary data indicate that the lp5 promoter is functional in cell suspension tissue. Analyses of sequence data and results of transformation studies are presented. This study will enable us to understand the molecular mechanism involved in stress tolerance and gene regulation in forest trees. Keywords: Pinus taeda, tater deficit stress, cDNA, promoter analyses, transformation.

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Author(s): M. A. Dilip L. Dias, V. Padmanabhan, S. Sen, M. E. Magallanes-Cedeno, John Cairney, Ronald J. Newton

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 1995