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Home → Publications → Tree Improvement and Genetics → Southern Forest Tree Improvement Conference → 23rd Southern Forest Tree Improvement Conference (1995) → Effects Of Acetosyringone, Ph And Concentration Of Agrobacterium Tumefaciens On Putative Transient Gus Gene Expression In Populus

Effects Of Acetosyringone, Ph And Concentration Of Agrobacterium Tumefaciens On Putative Transient Gus Gene Expression In Populus

Efficiency of transient GUS gene transfer mediated by Agrobacterium tumefaciens has been studied on Populus hybrid NC-5331 leaf explants. Included in this study were three factors which may affect the gene transfer efficiency: concentrations of acetosyringone (0 to 100 µM), dilution of the bacterium (25 and 50 times) and pH (5.5 to 6.4). All three factors were very important to achieve high efficiency gene transformation in the poplar. Interactions among the factors obviously existed. However, several tendencies were evident: 1) exogenous acetosyringone did not always enhance the gene transformation frequency, which was dependent on its concentration and the other factors; 2) acetosyringone preferred higher pH for higher transformation efficiency; 3) the most beneficial range of acetosyringone was between 25 and 75 µM, depending on the other factors; 4) dilution of the bacteria (overnight culture) 50 times, in most cases, resulted in higher transfer rate than the 25 times under the same conditions.


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Author(s): Xin Y. Li, Feng H. Huang

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 1995