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Home → Publications → Tree Improvement and Genetics → Southern Forest Tree Improvement Conference → 22nd Southern Forest Tree Improvement Conference (1993) → An automated approach to genetic mapping with Random Amplified Polymorphic DNA markers

An automated approach to genetic mapping with Random Amplified Polymorphic DNA markers

At least 10000 Random Amplified Polymorphic DNA (RAPD) reactions are required to complete a modest RAPD-based genetic mapping effort. Most molecular genetics laboratories are not equipped to handle this level of production in a time-efficient manner; and efforts to scale up are costly when considering equipment acquisition and personnel needs. We describe a RAPD laboratory of our own design that is capable of completing 2304 RAPD reactions per 2.0 technician-work-days and minimal space requirements (approximately 500 square feet).


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Author(s): L. S. Nelson, G. N. Johnson, M. L. Crawford, Warren L. Nance, C. Dana Nelson, R. L. Doudrick

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 1993

Section: Poster Session