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Genetic screening of tissue cultures for use in tree improvement programs

Plant tissue culture has many applications for forest tree improvement programs., However, genetic variation arising during the tissue culture phase of plant regeneration programs may limit the usefulness of in vitro propagation for certain forestry practices. Tissue cultures and regenerated plants should be routinely screened for abnormal nuclear conditions. Using two different tissue culture systems we regenerated buds from loblolly pine embryos. Microspectrophotometric analysis revealed that one system produced buds with a diploid nuclear DNA content but that the other system produced buds with nuclei containing abnormal amounts of DNA. Methods of genetic screening are compared. Additional keywords: Pinus taeda, microspectrophotometry, nuclear DNA.


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Author(s): Michael H. Renfroe, Graeme P. Berlyn

Publication: Tree Improvement and Genetics - North Central Tree Improvement Conference - 1983