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Home → Publications → American Chestnut Proceedings → 1982 → Liposome Encapsulated DSRNA For Cell-Free Transmission of Hypovirulence

Liposome Encapsulated DSRNA For Cell-Free Transmission of Hypovirulence

ABSTRACT.--Unilamellar lipid vesicles prepared by the ether injection method were used to capture dsRNA prepared from Endothia parasitica strain EP-113. The liposomes were fused with protoplasts of an E. parasitica virulent, methionine auxotrophic strain (EP-6). Protoplasts were regenerated on potato dextrose agar containing 0.55 M salt. Cell-free infection of protoplasts was also attempted using free dsRNA and dsRNA containing membrane-bound particles. Presence of the white phenotype was initially used as an indication of cell-free transmission. However, conversion experiments using EP-34(arg ) indicated otherwise and attempts to extract dsRNA from the white colonies were unsuccessful. The relatively high percentage of white colonies probably resulted from osmotic shock.


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Author(s): Neal K. Van Alfen, Dane R. Hansen

Publication: American Chestnut Proceedings - 1982