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Home → Publications → American Chestnut Proceedings → 1978 → Double-stranded RNA and Virus-like Particles in Endothia parasitica

Double-stranded RNA and Virus-like Particles in Endothia parasitica

Strains of Endothia parasitica were screened for the presence of double-stranded (ds) RNA. All virulent and physiologically normal strains lacked dsRNA. Many physiologically abnormal strains contained dsRNA and this included all strains designated hypovirulent. Hypovirulent strains contained one, two or three distinct size classes of dsRNA with estimated molecular weights ranging between 3.0 and 3.4 x 106. Double-stranded RNA from hypovirulent strains was transmitted by anastomosis to dsRNA free virulent strains which became hypovirulent. A French hypovirulent strain contained three dsRNA components and these were all associated with an unusual pleiomorphic club-shaped virus-like particle (VLP) which could be purified from mycelial pads. The VLP had a sedimentation coefficient between 115 and 190 S and a density of 1.28 in cesium chloride. A particle of this kind could not be purified by the same methods from an American dsRNA containing hypovirulent isolate.


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Author(s): J. Allan Dodds

Publication: American Chestnut Proceedings - 1978